gr 1  (Bio-Rad)

Journal: bioRxiv

Article Title: C26 and CT26 colorectal cancer models exhibit divergent cachexia phenotypes, intramuscular inflammation, and protein turnover signaling

doi: 10.64898/2026.04.21.719997

Figure Lengend Snippet: Sections of EDL (A-B) and SOL (F-G) were stained for immune cells including macrophages (MΦ) (CD68 + cells), M2-like MΦ (CD206 + cells), and polymorphonuclear neutrophils (PMNs) (Gr-1 + cells). Intramuscular immune infiltration was assessed by the quantification of CD68 + cells, CD68 + CD206 + cells, and Gr-1 + cells in EDL (C-E) and SOL (H-J) . Scale bar is set at 200 µm. The mRNA expression level of Cd68 (K) , Mrc-1 (L), Itgam (M) , Chmp2a (N) , Alox5 (O) , Alox15 (P), Alox12 (Q), and Ptgs2 (R) was determined by real-time quantitative reverse transcription PCR (RT-qPCR). Gene expression was normalized to the geometric mean of Vcp , Emc7 , and Gapdh . Western blot was used to examine the protein expression of leukocyte-type 12/15-LOX (S) . *P<0.05 for difference compared to healthy controls, #P<0.05 for difference between C26 and CT26 tumor-bearing mice.

Article Snippet: Primary antibodies were used to stain MyHC I (DSHB, BA-D5c, 1:100), MyHC IIa (DSHB, SC-71c, 1:100), MyHC IIb (DSHB, BF-F3c, 1:100), laminin (Abcam, ab7463, 1:100 or Novus Biologicals N-600-883, 1:100), Gr-1 (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:100 or Abcam, ab283654), and CD206 (Bio-Rad, MCA2235, 1:100).

Techniques: Staining, Expressing, Reverse Transcription, Quantitative RT-PCR, Gene Expression, Western Blot

Journal: bioRxiv

Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

doi: 10.64898/2026.04.21.719989

Figure Lengend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

Article Snippet: Primary antibodies used include MyHC type I [Developmental Studies Hybridoma Bank (DSHB), BA-D5c, 1:100], MyHC type IIA (DSHB, SC-71c, 1:100), MyHC type IIB (DSHB, BF-F3c, 1:100), eMHC (DSHB, F1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD206 (Bio-Rad, MCA2387, 1:50), and laminin (Abcam, ab7463, 1:200).

Techniques: Immunofluorescence, Staining, Labeling

Journal: bioRxiv

Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

doi: 10.64898/2026.04.21.719989

Figure Lengend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

Article Snippet: Primary antibodies used include MyHC type I [Developmental Studies Hybridoma Bank (DSHB), BA-D5c, 1:100], MyHC type IIA (DSHB, SC-71c, 1:100), MyHC type IIB (DSHB, BF-F3c, 1:100), eMHC (DSHB, F1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD206 (Bio-Rad, MCA2387, 1:50), and laminin (Abcam, ab7463, 1:200).

Techniques: Immunofluorescence, Staining, Labeling

Journal: bioRxiv

Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

doi: 10.64898/2026.04.21.719989

Figure Lengend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

Article Snippet: Primary antibodies used include MyHC type I [Developmental Studies Hybridoma Bank (DSHB), BA-D5c, 1:100], MyHC type IIA (DSHB, SC-71c, 1:100), MyHC type IIB (DSHB, BF-F3c, 1:100), eMHC (DSHB, F1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD206 (Bio-Rad, MCA2387, 1:50), and laminin (Abcam, ab7463, 1:200).

Techniques: Immunofluorescence, Staining, Labeling

Journal: Molecular Medicine

Article Title: Alpha-1 antitrypsin–glucocorticoid receptor axis: a new pathway in immune modulation

doi: 10.1186/s10020-026-01470-z

Figure Lengend Snippet: Endogenous AAT and GR co-localize in human PBMCs. Co-staining of adherent human blood PBMCs with anti-AAT ( red ) and polyclonal ( A ) or monoclonal ( B ) anti-GR ( green ) antibodies reveals co-localization of AAT and GR in the cytoplasm. Nuclei were stained with DAPI. Images were taken using a confocal laser scanning microscope (Olympus FluorView 1000) equipped with a 60× oil-immersion objective. Scale bars represent 20 μm

Article Snippet: Adherent cells were fixed with 4% paraformaldehyde (PFA) for 10 min at 37 °C, permeabilized with 0.05% Triton-X100 for 5 min and blocked with 5% BSA in PBS for 1 h. AAT was stained with monoclonal mouse-anti AAT antibody (Proteintech, dilution 1:200) and GR was stained with polyclonal rabbit anti-GR antibody (Thermo Fisher Scientific, dilution 1:100) or monoclonal rabbit anti-GR antibody (Cell Signaling, dilution 1:200).

Techniques: Staining, Laser-Scanning Microscopy